Coding

Part:BBa_K809331:Design

Designed by: Luohao Xu   Group: iGEM12_Shenzhen   (2012-09-26)


GAL promoter + YFP + SPtom40 + Toc75+ ADH1 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1224
    Illegal PstI site found at 1763
    Illegal PstI site found at 2282
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3068
    Illegal PstI site found at 1224
    Illegal PstI site found at 1763
    Illegal PstI site found at 2282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2506
    Illegal BamHI site found at 2933
    Illegal XhoI site found at 1526
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1224
    Illegal PstI site found at 1763
    Illegal PstI site found at 2282
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1224
    Illegal PstI site found at 1763
    Illegal PstI site found at 2282
    Illegal NgoMIV site found at 2105
    Illegal NgoMIV site found at 3074
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2642
    Illegal BsaI.rc site found at 1974
    Illegal SapI site found at 687
    Illegal SapI.rc site found at 1485
    Illegal SapI.rc site found at 2714


Design Notes

All parts should be linked correctly without nonsense mutation and frameshift mutation



Source

https://parts.igem.org/wiki/index.php?title=Part:BBa_K809601 https://parts.igem.org/wiki/index.php?title=Part:BBa_K809316 https://parts.igem.org/wiki/index.php?title=Part:BBa_K809209 https://parts.igem.org/wiki/index.php?title=Part:BBa_K165010

References